16s rna pyrosequencing  (Pyrosequencing Inc)

Journal: Clinics

Article Title: MICRObiota on BILIOpancreatic malignant diseases [MICROBILIO]: A systematic review

doi: 10.1016/j.clinsp.2022.100101

Figure Lengend Snippet: Reported studies limitations.

Article Snippet: Mei Q-X (2018), China , “In this study, our aim was to characterize the specific composition of the duodenal microbiota in pancreatic cancer patients using 16S ribosomal RNA (rRNA) pyrosequencing methods”. , The role of the duodenal microbiota in pancreatic head cancer cannot be ruled out, as the analysis of the microbiota based on LEfSe revealed small changes in relation to healthy control subjects..

Techniques: Biomarker Discovery, Bacteria, Control, Comparison, Sequencing

Journal: Clinics

Article Title: MICRObiota on BILIOpancreatic malignant diseases [MICROBILIO]: A systematic review

doi: 10.1016/j.clinsp.2022.100101

Figure Lengend Snippet: Experimental studies main results.

Article Snippet: Mei Q-X (2018), China , “In this study, our aim was to characterize the specific composition of the duodenal microbiota in pancreatic cancer patients using 16S ribosomal RNA (rRNA) pyrosequencing methods”. , The role of the duodenal microbiota in pancreatic head cancer cannot be ruled out, as the analysis of the microbiota based on LEfSe revealed small changes in relation to healthy control subjects..

Techniques: Control

Journal: Clinics

Article Title: MICRObiota on BILIOpancreatic malignant diseases [MICROBILIO]: A systematic review

doi: 10.1016/j.clinsp.2022.100101

Figure Lengend Snippet: Observational studies main results.

Article Snippet: Mei Q-X (2018), China , “In this study, our aim was to characterize the specific composition of the duodenal microbiota in pancreatic cancer patients using 16S ribosomal RNA (rRNA) pyrosequencing methods”. , The role of the duodenal microbiota in pancreatic head cancer cannot be ruled out, as the analysis of the microbiota based on LEfSe revealed small changes in relation to healthy control subjects..

Techniques: Control

Journal: Clinics

Article Title: MICRObiota on BILIOpancreatic malignant diseases [MICROBILIO]: A systematic review

doi: 10.1016/j.clinsp.2022.100101

Figure Lengend Snippet: Objectives and conclusions of studies.

Article Snippet: Mei Q-X (2018), China , “In this study, our aim was to characterize the specific composition of the duodenal microbiota in pancreatic cancer patients using 16S ribosomal RNA (rRNA) pyrosequencing methods”. , The role of the duodenal microbiota in pancreatic head cancer cannot be ruled out, as the analysis of the microbiota based on LEfSe revealed small changes in relation to healthy control subjects..

Techniques: Bacteria, Isolation, Control, Activation Assay, Sequencing, Probiotics

Journal: BMC Nephrology

Article Title: The gut microbiota profile of adults with kidney disease and kidney stones: a systematic review of the literature

doi: 10.1186/s12882-020-01805-w

Figure Lengend Snippet: Characteristics of included studies

Article Snippet: Shi 2014 [ ] , China , N = 52 n = 22 HD group Dialysis vintage: 6-40 months, Kt/V not reported. n = 30 reported ESKD not undertaking dialysis. eGFR, age, gender and ethnicity not reported. , N = 10 eGFR, age, gender and ethnicity not reported. , Details of sample collection not reported; Samples immersed in 90% alcohol and stored at -20°C , Excluded if taken in prior 3 weeks. , Medications not reported; Corticosteroids, statins, cytotoxic drugs not taken in prior 3 weeks. , SILVA , 16S ribosomal RNA gene Pyrosequencing (V1-V3 region) Sequencing platform not reported , ↑ α-diversity in HD group vs. controls (average OTUs/ species, p =0.044) and Chao1 index higher in three HD samples ( p -value not reported). β-diversity not reported between study groups. However, PCA plot of Unifrac data from selected samples at the genus level exhibited a large separation in the same three HD samples vs. the other selected samples ( p -value or statistical test not report). , Not reported.

Techniques: Medications, Sequencing, Control, Amplification, Real-time Polymerase Chain Reaction, Generated, Microarray, Sterility, Sampling, Transplantation Assay